I am sharing about a very scary virus infection. It causes hepatitis and has mortality rate of 30 percent. Quite scary huh!
We all heard about hepatitis B. But the motality rate of hepatitis B is not that high (0.5 - 1%). However this particular virus is somehow related to hepatitis B virus and it depends on the hepatitis B virus to infect the host.
Hepatitis D Virus
This interesting virus is the Hepatitis D Virus (HDV). HDV is a single-stranded RNA virus and it has HDAg as a enveloped protein (enclosing the RNA) and HBsAg (derived from hepatitis B virus) as its surface antigens. Without the HBsAg coating, HDV cannot infect, replicate or express on its own. The famility of HDV has not been identified.
Route of transmission and symptoms are similar to those of HBV. But it is more severe. It was reported that 70-80% of chronic HBV carriers with HDV superinfection develope chronic liver disease wuth cirrhosis.
As I mentioned HDV need HBV, thus the disease occur when HDV either superinfects the chronic HBV carriers or coinfect the person together with HBV. Symptoms are more severe in superinfection cases.
There is no vaccine for HDV and the best way to prevent it is to avoid risk behaviors. Immunization to HBV can avoid coinfection.
Okay...so much about the background and now I am sharing the laboratory diagnosis of HDV.
HDV is diagnosed serologically by detecting total antibodies to HDAg (delta antigen) by Competitive ELISA. The principle of the essay is as below. Enjoy....
Anti-HD present in the sample and labelled anti-HD antibodies compete for a fixed number of HDAg on the surface (sold-phase). The enzyme-labelled anti-HD gives colour after adding substrate, and the concentration of enzyme-labelled anti-HD can be worked out in relation to the O.D. reading. The amount of labelled anti-HD measured is inversly propotional to the concentration of anti-HD present in the samples.
OK. That's all for my posting. I hope you all enjoy it.
All the best for your SIP & MP yeah!