- Order the test by batch (by rack number). Note that each rack can only contain 5 sample tubes.
- Remove the caps of the sample tubes and make sure the appropriate barcode is pasted on it and the volume of the serum is sufficient. If not, the serum need to be aliquot out into a new test-tube with the patient barcode pasted on it.
- Place the sample tubes on the 1st rack ordered and place in the machine. Make sure the tubes sit in properly to prevent prop crashed.
- Place the last few tubes on the last rack ordered. In between any racks can be used, meaning that the racks that are used in between the two racks ordered will be tested for HIV.
- Then the machines will take in the racks one by one.
Automatic steps that are performed by the machine:
- 50ul of patient's serum will be aliquot out form the sample tube into a cuvette and incubate for 6 min at 37°C.
- Reagents will be aliquot into the cuvette and incubate again for 18min at 37°C.
- The cuvette will then wash with Wash 1.
- Another reagent will then be aliquot into the cuvette and incubate for 18min at 37°C.
- The cuvette will then wash with Wash 1 again.
- Acid Reagent and Base Reagent will then aliquot into the cuvette to initiate cheminluminescence reaction.
- Reactive is more that 0.9
- Non-reactive is less that 0.9
- Repeat the testing again. Spin down the sample tube at 10000rpm for 10 min, aliquot the serum out into a new test tube with appropriate barcode, then rerun in another machine.
- If repeat test is negative, report as "Not Detected".
- If repeat test is positive, call clinic to inform Doctor that Western Blot need to be done for confirmation.
- If Western Blot is negative, attached the report to screening report and send directly to Doctor.
- If Western Blot is positive, reports need to be notified to referring Doctor via phone and the report will be sent directly to the Doctor in a confidential envelope and CDC will be informed.
- The reactive samples will be sent to hospital for Western Blot test.
No wonder Ye Tun, you tell me that you received samples from my lab. You all should know where the Western Blot is done by now.... Hope my post helps you all to understand HIV testing. Feel free to ask me questions.
Lizzie Chew, TG01, 0503194C